A Radioreceptor Assay for Insulin: Direct Measurement of Dog Pancreatic Vein Serum Insulin
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概要
- 論文の詳細を見る
A simple radioreceptor assay for insulin using rat liver membranes as receptor sites, with sufficient specificity, precision, and sensitivity to detect 10ng or 276μU/m<I>l</I> of serum insulin, has been developed. In the presence of standard porcine insulin at the concentration of 1.0ng/tube, approximately 8% of <SUP>125</SUP>I-porcine insulin was bound to the plasma membranes and ninety-five per cent of this binding was inhibited by 1.0μg of standard insulin per tube. Four animal insulins inhibited the binding of <SUP>125</SUP>I-insulin while ACTH, glucagon, human growth hormone, and oxytocin were inert. Insulin values in dog pancreatic vein sera obtained during and after glucose loading and measured by the present radioreceptor assay agreed well with immunoreactive insulin. The ratio of IRI to the measurement by radioreceptor assay was 1.09±0.18 for the same sera.
- 社団法人 日本内分泌学会の論文
著者
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Kosaka Kinori
The Third Department Of Internal Medicine University Of Tokyo
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OHSAWA NAKAAKI
The Third Department of Internal Medicine, University of Tokyo Faculty of Medicine
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KABUTO MICHINORI
Department of Human Ecology School of Hgalth Sciences, Faculty of Medicine, University of Tokyo
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SUZUKI KAZUO
The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo
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