A Rapid Method for the Separation of Rat Pancreatic Islets from Collagenase-Digested Pancreas using Percoll
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概要
- 論文の詳細を見る
The isolation of pancreatic islets from collagenase-digested Wistar rat pancreas was shown by the sedimentation method at a unit gravity using Percoll solution with a density of 1.041g/m<I>l</I>. The density of digested exocrine tissues was in the range of 1.013-1.041g/m<I>l</I>, while that of purely isolated islets was in the narrow range of 1.066-1.075g/m<I>l</I>. More than a hundred islets were obtained freely from each rat pancreas without any gross contamination of digested exocrine tissues. A significant increase was observed in glucose-stimulated insulin release from islets isolated with Percoll in the same pattern as that without Percoll.<BR>In addition to the well preserved morphology and function of pancreatic islets isolated by Percoll, the simplicity of the technique strongly commends the usefullness of this method.
- 社団法人 日本内分泌学会の論文
著者
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Yamamoto Takashi
The First Department of Internal Medicine, Shiga University of Medical Science
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SOJI Tsuyoshi
The First Department of Anatomy, Nagoya City University Medical School
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ASANO TAKASHI
The First Department of Internal Medicine and the Department of Anatomy, School of Medicine, Fukuoka University
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MORI AKIRA
The First Department of Internal Medicine and the Department of Anatomy, School of Medicine, Fukuoka University
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KUMAI CHIE
The First Department of Internal Medicine and the Department of Anatomy, School of Medicine, Fukuoka University
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OKUMURA MAKOTO
The First Department of Internal Medicine and the Department of Anatomy, School of Medicine, Fukuoka University
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SOJI TSUYOSHI
The First Department of Internal Medicine and the Department of Anatomy, School of Medicine, Fukuoka University
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YAMAMOTO TAKASHI
The First Department of Internal Medicine and the Department of Anatomy, School of Medicine, Fukuoka University
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