A CRITICISM OF THE SWEAT'S METHOD FOR THE ESTIMATION OF CORTICAL HORMONES IN PLASMA

概要

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A fluorometric method in combination with the silica gel column chromatography by Sweat for the estimation of cortical hormones was reinvestigated under a slight modification by the author. And with specimens of human being and some animals, normal values were given by this method.<BR>The fluorogenic substances contained in the fractions of human, rat and guinea-pig plasma, were analysed further by the paper chromatography, and the following results were obtained;<BR>a) In human being the so-called Cpd F-like substance appears to indicate practically the level of hydrocortisone itself, but in the so-called Cpd B-like substance, somewhat other substances besides a small amount of corticosterone are contained.<BR>b) In rat plasma corticosterone made the chief component of the so-called Cpd B-like substance in which the presence of a small amount of Reichstein's Substance S was also detected.<BR>c) In guinea-pig plasma hydrocortisone, which is detectable in the Cpd Ffraction, appears to be the major cortical hormone; corticosterone and the other known active principles were not found in the Cpd B-fraction.
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