Qualitative and Quantitative Changes in Plasma Luteinizing Hormone (LH) under Stimulation by Intravenous Infusion of Synthetic Luteinizing Hormone-Releasing Hormone (LHRH) in Japanese Monkeys (<I>Macaca fuscata</I>) as Assessed by Electrofo-cusing
スポンサーリンク
概要
- 論文の詳細を見る
Using 5 adult Japanese monkeys (<I>Macaca fuscata</I>) of both sexes, the qualitative and quantitative changes in plasma LH caused by LHRH administration were examined before and after castration. Five hundred μg synthetic LHRH was infused for 2hrs and blood samples were obtained every 15min for 5hrs under ketamine hydrochloride anesthesia. Plasma LH levels were determined by an <I>in vitro</I> bioassay using the mouse testis interstitial cell preparation. The LHRH infusion induced a biphasic release of in intact animals of both sexes and in castrated females but not in castrated males. The biphasic pattern of LH release was characterized by an initial acute rise followed a plateau or a fall and the consecutive second rise after a long latent period.<BR>Aliquots of plasma samples obtained during 30-90 (corresponding to the initial rise) and 120-210min (corresponding to the second rise) after the initiation of LHRH infusion were pooled separately (plasma pools I and II, respectively) and subjected to isoelectrofocusing (IEF) fractionation. The IEF fractionation was performed in ampholine-sucrose gradient, pH range 3.5-10, under a constant electrical power supply (3W, 700V) for 48hrs at 4°C. After the completion of the IEF fractionation the LH activity in each fraction was measured by the <I>in vitro</I> bioassay. The IEF fractionation separated plasma LH into 6 distinct species in terms of pI values. When the IEF profiles of plasma pools I and II in intact animals of both sexes were compared, the increase and the decrease in acidic to neutral LH and alkaline LH, respectively, were consistent and significant in plasma pool II. Changes of the same kind were further exaggerated when the IEF profiles in castrated animals were compared with those in non-castrated ones.<BR>The acidic LH may represent the LH species newly synthesized and not well processed. The LH species possessing pI values of 7.5-8.5 is likely to be the mature type of LH exhibiting high biological potency. The LH species migrating in the high alkaline region seems to be the LH of over-maturation.
- 社団法人 日本内分泌学会の論文
著者
-
Suginami Hiroshi
Department Of Obstetrics And Gynecology Clinical Research Unit Kyoto National Hospital
-
HAMADA Katsuyuki
Department of Obstetrics and Gynecology Ehime University School of Medicine
関連論文
- Laparoscopic Surgery for Endometriosis : A Long Term Follow-Up
- Bestatin, an Aminopeptidase Inhibitor, Promotes Follicular Growth and Ovulation Suppressed by Stress in Mice
- Allelic Loss of Chromosome 16q in Endometrial Cancer : Correlation with Poor Prognosis of Patients and Less Differentiated Histology
- Association of Replication Error Positive Phenotype with Lymphocyte Infiltration in Endometrial Cancers
- A Case of Laparoscopic Complication : Injury of the Left Common Iliac Vessels and Subsequent Acute Compartment Syndrome of the Left Leg
- Biological and Immunological Characterization of Human Luteinizing Hormone Discharged in a Pulsatile Fashion in the Normal Menstrual Cycle
- Biological and Immunological Characterization of Human Luteinizing Hormone Discharged by the Stimulation of Synthetic Luteinizing Hormone-Releasing Hormone (LH-RH) in Normal and Anovulatory Women
- A Trial for the Improvement of the in vitro Bioassay for Human Luteinizing Hormone
- Measurements of Human Luteinizing Hormone in Plasma by in vitro Bioassay and by Conventional and Improved Radioimmunoassays
- Qualitative and Quantitative Changes in Plasma Luteinizing Hormone (LH) under Stimulation by Intravenous Infusion of Synthetic Luteinizing Hormone-Releasing Hormone (LHRH) in Japanese Monkeys (Macaca fuscata) as Assessed by Electrofo-cusing
- Meta-analysis of genome-wide association scans for genetic susceptibility to endometriosis in Japanese population