イオン交換クロマトグラフィーによるベルベリンとアトロピン,スコポラミンとの分離
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概要
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Attempts have been made to separate berberine, atropine, and scopolamine by use of weak cation-exchange resin (Duolite CS-101, 100200 mesh).<BR>An aqueous solution containing a mixture of berberine hydrochloride, atropine sulfate, and scopolamine hydrobromide was passed through a column of Duolite CS-101 (NH<SUB>4</SUB>-form) for adsorption of the alkaloids. A portion of scopolamine was eluted unadsorbed and the rest of it was liberated by washing the column with water. The column was then treated with 0.2<I>N</I> NH<SUB>4</SUB>Cl for liberation of atropine and finally with 1<I>N</I> Na<SUB>2</SUB>CO<SUB>3</SUB> for liberation of berberine.<BR>Each alkaloid thus liberated was determined by the amperometric titration.<BR>The recovery was above 85% for atropine and scopolamine and above 95% for berberine.