イオン交換クロマトグラフィーによるベルベリンとストリキニンの分離
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概要
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The separation of berberine and strychnine was attempted by using a weakly acid cation exchanger. By comparing the separability of berberine and strychnine on each of the resin forms, R-COOH, R-COONa and R-COONH<SUB>4</SUB>, the form R-COONH<SUB>4</SUB> was found to be the most suitable for the purpose.<BR>After the alkaloids were absorbed by passing a mixture of strychnine nitrate and berberine hydrochloride (each 2050mg) through a column of Duolite CS-101 (R-COONH<SUB>4</SUB>), the column was washed with a small quantity of water, and strychnine only was eluted with NH<SUB>4</SUB>Cl (1<I>N</I>).<BR>After that, berberine was eluted with Na<SUB>2</SUB>CO<SUB>3</SUB> (1<I>N</I>). The separation was complete and quantitative.<BR>Berberine and brucine were also separated by the similar means with good results.