セファデックスカラムによる血清ビリルビンの分画定量法
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概要
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By an elution of serum on a column of Sephadex LH 20 with 35 (vol) % ethanol as the eluent, glucuronide bilirubin in the serum comes out at first accompanying with serum protein and free bilirubin comes out separately afterwards. The separation is quantitative.<BR>As the specificity of this methodhas been ascertained by making use of the difference in solubility of the two types of bilirubin--<I>i</I>. <I>e</I>., the free type is waterinsoluble and organic solvent-soluble while the glucuronide type is water-soluble and organic solvent-insoluble-, the proposed method is more reliable than conventional ones.
- 社団法人 日本分析化学会の論文