サイクラミン酸ナトリウムの簡易迅速定量法

概要

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A simple and rapid colorimetric method was proposed for the determination of sodium N-cyclohexyl-sulfamate(sodium cyclamate). Sodium cyclamate formed cyclohexylamine by the reaction with dimethylsulfoxide in acidic medium of sulfuric acid and this cyclohexylamine gave a colored product with quinhydrone. Three milliliters of dimethylsulfoxide and 0.5 ml of 0.5 N sulfuric acid were added to the test solution(containing 1001000 μg/ml). The mixture was heated in a boiling water bath for 60 minutes and, after cooling, was neutralized with sodium hydroxide. The mixture was adjusted to pH 12.0 with a buffer solution and extracted with chloroform. The extract was washed with a Kolthoffs buffer(pH 12.0). Ethanol and quinhydrone reagent were added to the chloroform solution. The mixture was warmed and its absorbancy was read at 495 mμ.
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