ビタミンB<SUB>12</SUB>の生合成経路の<SUP>13</SUP>C-NMRによる研究
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概要
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The biosynthesis of corrin was examined in a new light using <SUP>13</SUP>C-labeling and NMR techniques which added a new dimension to the chemists armamentarium and opened a new era in the study of the biogenesis of the complicated cobalamin. The use of <SUP>13</SUP>C-FTNMR has led to the observation that 8 molecules of [2-<SUP>13</SUP>C] ALA are incorporated into vitamin B<SUB>12</SUB>, [5-<SUP>13</SUP>C] ALA labels seven only of the carbon atoms cyanocobalamin, i.e. amino methyl groups of ALA is lost the process. It has also been confirmed that seven of the methyl groups of B<SUB>12</SUB> are derived from [<SUP>13</SUP>CH<SUB>3</SUB>] -methionine and further that the chirality of the gemdimethyl grouping at C<SUB>12</SUB> labelled with [<SUP>13</SUP>CH<SUB>3</SUB>] -methionine is R. Four [8-<SUP>13</SUP>C] -PBG were incorporated into V.B<SUB>12</SUB> giving rise to 4 centers of enriched carbons. Mutiply-labelled urogenIII (<SUP>13</SUP>C, <SUP>14</SUP>C and <SUP>3</SUP>H) has been used to show that incorporation takes place without randomization. A sequence for corrin synthesis from urogenIII is presented. Also this review is shown more recently results in the corrin pathway.
- 社団法人 有機合成化学協会の論文