アセチレン還元法による窒素固定速度の現場測定法 (水圏における窒素固定に関する微生物学的研究-5,6-〔英文〕)

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For the purpose of estimating the in situ rate of nitrogen fixation which proceeds in aquatic environments, some modification has been made in the acetylene reduction method by the authors. The analysis of ethylene formed from acetylene was carried out by a gas chromatograph which was equipped with a hydrogen flame ionization detector and a column (750×3mm, 100°C) of activated charcoal coated with 1.5% squalane. Nitrogen was used as carrier gas at a flow rate of 100mL/min. The procedure for the in situ measurement of acetylene reduction was as follows: A glass bottle (150mL) fitted with a rubber stopper and a stop cock was used for the reaction vessel. After incubating the bottle containing the water sample and the gas mixture of acetylene, argon and oxygen at the in situ position of the sample for 30 or 60 minutes, ethylene produced in the bottle was absorbed into a solution of mercuric perchlorate using the ethylene trapping system.
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