軟体動物成分に関するポーラログラフ的研究-VI : ‘するめ’肉からポーラログラフ還元波塩基成分の単離について

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In the former papers1-5), studies have been carried out on the polarographically reducible and ultraviolet light-absorbing base (abbreviated as PRB) which is peculiar to the muscle or tissues of mollusca and crustacea. In this experiment, isolation of the PRB was tried from the extracts of dried squid muscle. The fraction precipitable with phosphotungstic acid from the above extracts was chromatographed on a cation exchanger column by the method described in the former papers4, 5). Other bases and amino acids were removed by adsorbing the PRB with active charcoal or by precipitating with ammoniumreineckate or alcoholic saturated picric acid solution (in Figs. 1, 2 and 3; in Table 3). The PRB was positive in DRAGENDORFFs reaction (in Table 1). Then, the PRB was compared with commercial specimens of trigonelline (N-methyl nicotinic acid betaine, an isomer of homarine), purines or pyrimidines, nucleosides and 5-hydroxy tryptamine4) by paperchromatographic technique (in Table 2), to show the Rƒ values roughly resembling to those of trigonelline. The melting point of the PRB chloraurate was 188-189°C. Thus, the PRB seemed to be homarine (N-methyl picolinic acid betaine) isolated for the first time from lobster by HOPPE-SEYLER8, 9).
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