魚類血液の酵素に関する研究-1-

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During the last ten years, the problem of an esterase specific for acetylcholine has attracted the attention of many investigators and has got a satisfactory answer. Cholinesterase (ChE) preparation has been made from tissue and body fluid of various vertebrates by AUGUSTINSSON in comparative enzymology. Study on the fish blood ChE, however, has not been almost made. Present work is concerned with the preparation and purification of ChE in serum or erythrocyte of fish blood, and their chemical properties. The results obtained were as follow: 1) As shown in Table 1 and Fig. 1, serum ChE solution was highly purified from carp blood by the fractionation with ammonium sulphate; and this solution was capable of showing the activity of 2.725 (hydrolysis-ACh-mg/protein-mg/hour). 2) Erythrocyte AChE solution was purified from the carp blood by the purification shown in Fig. 2; and this solution was capable of showing the activity of 5480 hydrolysis-ACh-mg/protein-mg/hour). 3) The opitimum pH of ChE in serum or erythrocyte of carp was about 7.8, and their opitimum temperature were 27.5°C. 4) Activity-pS-curves of serum ChE were S-type; while those of erythrocytes were Bell-type with pS 2.5 of optimum substrate-concentration for ACh.
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