魚類食品におけるボツリヌスE型菌の発肓と毒素産生に関する研究-II : ボツリヌスE型菌の栄養要求について

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The nutritional requirements of Cl. botulinum type E, strains IWANAI & TENNO, which had already been isolated in Japan, were studied and compared with those of other serological types A and B. It was found that type E differed from the latter two types in the following two nutritional patterns. 1) The requirement of fermentable sugars as energy source for growth: type E organisms were unable to grow in media free from sugars (Table 1), whereas both types A and B grew well in such media. 2) The requirement of some unidentified peptides as growth-supporting factor: type E organisms failed to grow in a partially defined medium supplemented with various vitamins, when casamino acid was used as nitrogen source; however, they grew well when casitone was substituted for casamino acid. In addition, trypsinized preparations of various proteins also promoted growth. This growth-supporting factor in casitone or trypsin digest of proteins may be a kind of peptide containing γ-glutamyl group, but its identity with “Strepogenin” was not ascertained.
公益社団法人日本水産学会の論文