餌料微生物特に海産原生動物の培養と保存

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Recently, considerable attention has been paid to free-living marine protozoa as. food for the rearing of marine animal larvae. However, even after a long time of experimentations, many different problems on the collection, isolation and cultivation of these free-living marine protozoa still remain to be settled. The present paper reports the preliminary studies made on the cultivation and simple preservation methods for marine protozoa. The method aims to make it possible to.supply food organisms whenever it is needed. The writers succeeded in the cultivation of five species of free-living marine protozoa (four species of ciliata and one of flagellata) isolated from marine algae, detritus and sea water, collected from the tidal zone around Aburatsubo Bay, near Misaki in Kanagawa Prefecture. A culture medium added 20 p. p. m. glucose to Allen-Miquel-Nelson Solution seemed to the most suitable one for the culture of marine protozoa. However, for the cultivation of flagellata, the medium when aglae-extract or dried activated sewage-sludge-extract added showed better results. The writers also succeeded in the encystment of cultivated protozoa by their new filteration method of which two factors, desiccation and crowding, may have contributed to the cyst formation of their cultivated organisms (Fig. 2). The filteration method may be generally suitable for the encystment of marine protozoa. Still more, this method is not only useful for the encystment of the protozoa, but also useful for the concentration and desiccation of the organisms. In our laboratory, five cultivated protozoa, preserved in a special form at room temperature for 10 months, have been observed to show normal excystments. The cysts were preserved in special "cyst powder" form, i.e. a mixture of cyst and a sufficient quantity of filter substances containing alumina and gypsum, by the method mentioned above.
社団法人日本水産学会の論文

餌料微生物特に海産原生動物の培養と保存

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