Chemical Studies of the Proteins of Feeding-stuffs:I. On the Distribution of Methionine
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概要
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Methioninc, a sulfur containing amino acid which was discovered by MUELLER (1921), has been found to be present in casein, egg-albumin, edestin, wool (?), gelatine (?), fibrin, protein of rice-bran, and brewery yeast, by several authors, such as MUELLER (1923), ODAKE (1925), DU VIGNEAUD and MEYER (1931), and PIRIE (1932). BAERESTEIN (1932) determined the methylthiol group, characteristic of methionine, in several proteins, and assumed that methionine is widely distributed in many kinds of proteins. Recently, JACKSON and BLOCK (1931, 1932), T. E. WEICHSELBAUN, M. B. WEICHSELRAUN and STEWART (1932) reported that the methionine, like cystine, is capable of unmistakably stimulating growth in albino rats subsisting on a basal diet poor in cystine. Nevertheless, as far as we know, this amino acid has not yet been isolated in the proteins of feeding-stuffs such as muscle, silk-worm pupa, and soy-bean. The present author isolated methionine in crystalline form from these feeding-stuffs by Mueller's method, slightly modi-fied by ODAKE, and identified it by elementary analysis both of the free amino acid and of its derivatives, namely copper salt and α-naphthyl-isocyanate, The yield of free methionine from the proteins of sardine muscle, whale muscle, silk-worm pupa, and soy-bean was 0.52, 0.37 0.43, and 0.08% respectively; that is, from moisture- and ash-free coagulable protein. Contrary to the result of ODAKE. methionine was isolated with certainty, though in a small amount, from the soy-bean protein. Furthermore, it was found that the methionine fraction isolated from silk-worm pupa only was largely contaminated with glutamic acid. Although only a few kinds of protein were investigated in this present study, it might perhaps be presumed that metbionine would be generally wide-spread in proteins, especially in those of animal origin.
- 社団法人 日本水産学会の論文
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関連論文
- Chemical Studies of the Proteins of Feeding-stuffs:I. On the Distribution of Methionine
- Chemical Studies of the Proteins of Feeding-stuffs:IV The Comparison of Digestibility by the Proteolytic Enzyme Contained in Eel's Viscera