Chemical Studies of the Proteins of Feeding-stuffs:IV The Comparison of Digestibility by the Proteolytic Enzyme Contained in Eel's Viscera
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The digestibility of the proteins of feeding-stuffs, which were dealt with in the preceeding report1), by the proteolytic enzyme contained in viscera of eel (Anguilla japonica) has been determined by measuring the amino nitrogen and the soluble nitrogen yielded during the digestion. The enzyme was prepared as follows: the internal organs, including stomach, intestine, liver, free from connective tissues, were dehydrated by acetone; the dried material thus obtained was extracted overnight with ten volumes of an equal mixture of M/5 phosphate (pH 7.3) and glycerine and then centrifuged. The digestion was carried out at 37°C and pH 7.3 since this condition was determined by OYA's experiment as the optimum for the protease of eel's spleen. The enzyme reaction was stopped by adding a concentrated trichloracetic acid solution sufficient to give a final concentration of 3 per cent. No difference in the yield of amino nitrogen during the digestion was observed among the three proteins as is shown in Table 1. However, judging from the increased amount of nitrogen soluble in 3 per cent trichloracetic acid, the protein of soy bean was digested more easily than the other proteins, i. e. the protein of silk-worm pupa and of sardine (Table 2). In Table 1 and Table 2 the first column shows the digestion period in hours; the second presents the experiments on the protein of sardine, the third on the protein of silk-worm pupa, and the fourth on the protein of soy bean respectively.
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関連論文
- Chemical Studies of the Proteins of Feeding-stuffs:I. On the Distribution of Methionine
- Chemical Studies of the Proteins of Feeding-stuffs:IV The Comparison of Digestibility by the Proteolytic Enzyme Contained in Eel's Viscera