STUDIES ON THE PRODUCTION OF BLACK PEARLS BY IRRADIATION WITH RADIOACTIVE-RAYS-I:CHANGES OF MANGANESE CONTENTS IN TISSUES OF PEARL OYSTERS CAUSED BY MANGANESE ADMINISTRATION

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1. This study was carried out to prove a hypothesis that the coloration of pearls by irradiation with radioactive-rays may be caused by growing a colored material which was produced from the manganese compound contained in pearls. 2. To obtain marine pearls containing rich amounts of manganese, three adminis-tration methods of manganese, that is, the manganous chloride-sea water immersion method, manganous carbonate paste injection method, and manganese nucleus insertion method were comparatively investigated by measuring the changes of manganese content in the various tissues of pearl oysters. 3. It is estimated that the concentration of manganese in the mantle required to obtain pearl oyster shell having a level of manganese content similar to that of fresh-water mussels should be kept at about 135 to 610γ per g of dry mantle. Such a concen-tration of manganese was obtained by the manganous carbonate injection method (about 100 to 1, 000γ for 5 days) and the manganese nucleus-insertion method (about 30 to 40γ for 6 days).
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