Stimulus-Transcription Coupling can be studied by in situ Hybridization of Brain Slice Preparation. Selective c-fos Expression in Hippocampal CA3 Pyramidal Cells.
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概要
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Slice was prepared from the rat hippocampus and subjected to in situ hybridization of c-fos and other mRNAs after electrical recording of the neural activities. Anesthesia of the animal before dissection and supplementation of the medium with vitamins and amino acids improved the condition for mRNA expression. Low concentration of kainic acid induced both the epileptiform discharge and c-fos mRNA in CA3 pyramidal cells.
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著者
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Obata Kunihiko
Laboratory Of Neurochemistry Department Of Molecular Physiology National Institute For Physiological
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SEKINO Yuko
Laboratory of Neurochemistry, National Institiute for Physiological Sciences
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OBATA Kunihiko
Laboratory of Neurochemistry, National Institiute for Physiological Sciences
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HASHIMOTO Takanori
Laboratory of Neurochemistry, National Institiute for Physiological Sciences
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