Cytoplasmic Movement in Spirogyra during and after Centrifugation.

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The mode of dislocation of the cell organelles in Spirogyra was observed while the algal filaments were being centrifuged at 600rpm (40g). When all the chloroplasts were packed at the centrifugal end of the cell together with the nucleus and most of the cytoplasm, a large clear area appeared in the centripetal end where only a thin layer of cytoplasm was left beneath the plasma membrane. Active movement of tubular organelles (endoplasmic reticulum, ER) was clearly seen in this area. With rhodamine-conjugated phalloidin, distinct fibrous structures (bundles of F actin) showed up running more or less longitudinally or transversally to the cell axis. By staining with DiOC6(3), a faint fluorescent image coinciding with that of the moving tubular organelles became recognizable but only for a few seconds after blue light irradiation before it was rapidly transformed into a polygonal structure. The formation of the polygon was reversible, but it did not present the normal state of the ER. The movement of the tubular ER is supposed to be caused by the interaction of the cortical F actin bundles and putative myosin-like protein(s) attached to the ER.
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