Immunochemical analysis of the species specific glycoprotein of the human red cell membrane.

概要

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Specific antiserum for human red cells was prepared from rabbit antihuman red cell serum by absorbing with red cells of horse, hog, dog and Japanese monkey. This antiserum proved specific for human red cells regardless of blood types ABO and MN demonstrated in hemagglutination test. A glycoprotein was extracted with lithium diiodosalicylate (LIS) and isolated through chromatographic procedures from human red cell membranes and the substance showed inhibitory activity for the specific antiserum as well as for anti-M and anti-N sera. The isolated glycoprotein corresponded to PAS-1 on SDS-PAG electrophoresis. The inhibitory activity of the PAS-1 for the specific antiserum was lost after the substance was treated with sialidase for 24 hr. A moderate reduction of the inhibitory activity for anti-M and anti-N sera but not for the specific antiserum was seen after the treatment for 1 hr. These results indicate that different structures involving sialic acids in the PAS-1 molecule may be responsible for the different susceptibilities to sialidase and these structures may reflect different antigenic determinants.
久留米大学医学部 The Kurume Medical Journal 編集部の論文