:Role of cytophilic anti-tumor antibodies in indirect MAI response of patients with HCC

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概要

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• The reactive cell in micro-leukocyte adherence inhibition (micro-LAI) assay was investigated in patients with HCC, using extract of human HCC cell line, "HCC-M" as specific antigen. Monocytes exhibited obvious reactivity among mononuclear cells. Separated monocytes from peripheral blood of patients with HCC also showed reactivity in the micro-LAI assay. For the purpose of analyzing effect of serum factor on the monocyte adherence inhibition, Indirect MAI assay Was perfomed. After healthy donor's monocytes were incubated with sera from patients With HCC, the micro-LAI assay was done. As a result, 11 out of 21 sera from patients with HCC showed positive reactivity in Indirect MAI assay, This "arming" factor of serum was involved in IgG fraction. Furthermore Antibody-Dependent Monocyte-Mediated Cytotoxicity (ADMC) assay was studied by measuring the lysis of HCC, M by healthy donor's monocytes preincubated with sera from patients with HCC. Sera from patients with HCC who had a positive Indirect MAI response increased the cytotoxicity of monocytes to HCC-M as compared with those from healthy controls. These results suggested that in some patients with HCC, there were circulating cytiphilic anti-tumor antibodies which participated in MAI response and ADMC to HCC.

• 社団法人 日本肝臓学会の論文

著者

• 熊谷 直樹

  慶応義塾大学医学部内科

• 熊谷 直樹

  慶応義塾大学医学部内科学教室

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• :Role of cytophilic anti-tumor antibodies in indirect MAI response of patients with HCC

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