Characterization of sinusoidal endothelial cells isolated from rat liver and the interrelation between cultured endothelial cells and hepatocytes.

概要

論文の詳細を見る
Sinusoidal endothelial cells (SECs) and hepatocytes were isolated from a same rat liver by a collagenase digestion technique and centrifugal elutriation rotor. SECs formed branching network on non-collagen coated culture dishes and cobblestone appearance on type-1-collagen coated dishes. SEC presented characteristic fenestrae referred to as sieve plates in situ. The fenestration appeared as labyrinth-like structure, but not tube-like. Cultured SECs on collagen gel presented vascular-like space. In co-culture of hepatocytes and SECs, SEC extended their cytoplasmic processes around the hepatocytes and formed an interecellular space between two cells similar to the space of Disse. Collagen gel was useful in investigating the three-dimensional structure of the cultured cells.
社団法人日本肝臓学会の論文