Analysis of mechanism of the effect of partial hepatectomy on enhanced growth of liver cancers in rats: Experimental analysis of the mechanism of recurrence after liver surgery in human liver cancers.

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In order to reveal the mechanism of postoperative recurrence of liver cancers in humans, we attempted to analyze the mechanism of the enhanced growth of liver cancers following partial hepatectomy (PH), from the standpoints of changes in host postoperative immunity, expression of cytosol estrogen receptors (E2R) in liver cancer cells and liver regeneration factor, using WKA/H rats and the hepatocellular carcinoma KDH-8 cell line induced by 3'-Me-DAB. The changes in postoperative immunity (NK activity and Blastogenesis) did not correlated with changes in liver cancer growth. Although serum estradiol (E2) increased significantly after PH, E2R was not detected in the KDH-8 cells used in this experiment. Serum was obtained from healthy rats 24 hours after PH, and 20mg of serum, as calculated from total protein, was eluted into 50 fractions by high performance liquid chromatography (column; TSK G3000SW). When we investigated which fractions stimulated the growth of both primarily cultivated hepatocytes and KDH-8 cells, only the fraction Fr. 30, the molecular weight of which was over 100Kd, enhanced both. Furthermore, we performed an in vivo assay to determine the number of days needed for tumor appearance: PHs were done 2 months, 5 days and 1 day before, at the same day of, and 1 day and 5 days after KDH-8 cell (500 cells/100μl) inoculation (sc). We also observed from in vivo tests that liver regeneration factor enhanced the growth of liver cancers.
社団法人日本肝臓学会の論文

Analysis of mechanism of the effect of partial hepatectomy on enhanced growth of liver cancers in rats: Experimental analysis of the mechanism of recurrence after liver surgery in human liver cancers.

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