Rat hepatocyte/in situ nick translation assay as a screening model to detect chemical carcinogens.

概要

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Primary cultured hepatocytes have been applied to in situ nick translation method to detect DNA single-strand breaks by chemicals. Dimethylnitrosamine (DMN) and aflatoxin B1 (AFB1) were used as well-known hepatocarcinogens and lysophosphatidylcholine (LPC), phospholipase A2 (PLA2) and lithocholic acid (LCA) were used as suspected carcinogens. DNA damages by DMN, AFB1 and LCA could be detected at higher than 2×10-5M, 1.6×10-7M and 5×10-7M respectively, whereas LPC and PLA2 did not induce DNA damages. Compared with unscheduled DNA synthesis (UDS) and alkaline elution technique, this system is far more sensitive than the former and more simple than the latter. Thus, this assay can be used as a screening model which possesses metabolic activation systems for carcinogenicity of many other chemicals.