ラット肝の部分切除後の肝再生過程における肝小葉の経時的変化.
スポンサーリンク
概要
- 論文の詳細を見る
The present study was performed to elucidate the actual regeneration process occurring after partial hepatectomy of the rat liver. Five-week-old male SD rats were subjected to two-thirds partial hepatectomy (PH), and vascular and lobular changes were investigated by means of an injection technique. In addition, cellular kinetics were studied with intraperitoneal administration of BrdU, and S-phase cells were detected immunohistochemically. Elongation and newly formed branching patterns of terminal portal, arterial and hepatic vein branches were recognized by the 3rd experimental day and increased thereafter. 28 days after PH, the terminal portal and hepatic vein branches of operated rats were recognized as 7th order, in clear contrast to the constant finding in control rats of 5th order during the present study. Dimensions of hepatic lobules in PH rats were larger than that of control animals during the entire experimental period of four weeks. Even on the 28th experimental day, the enlarged hepatic lobules of PH rats had not been restored to the size of control animals. The peak incidence of BrdU positive hepatocytes was noted 24 hours after the PH, with preferential localization in the peripheral zones of hepatic lobules. A relatively large number of BrdU positive periportal cellular elements and littoral cells were also detected on the 3rd experimental day. Special stress was laid on the important roles played by newly formed circulatory pathway in lobular hypertrophy and new lobular formation after PH.
- 社団法人 日本肝臓学会の論文