ヒト肝細胞癌および実験肝癌における癌細胞の蛋白合成能に関する研究--アルブミン合成能について
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Hepatocellular carcinoma (hepatoma) cells retain certain synthetic functions of normal hepato-cytes. The present investigation studied albumin synthesis in human autopsy hepatoma tissue and 3'-methyl-diazobenzene induced rat hepatoma tissue. Human tissue was stained for albumin by per-oxidase anti-peroxidase and diaminobenzadine. Rat tissue was subjected for the same staining pro-cedure and also <SUP>14</SUP>C-L-leucine radioprecurssor uptake by the antiserum-precipitated albumin frac-tion and albumin fraction measurement by the Laurell-Rocket method. <BR>The following results were obtained: (1) positive staining of albumin was observed in human hepatoma cells as well as in hepatocytes tissue from 24 patients (77.4%) of a total of 31 patients with hepatoma and 87.1% of hepatoma tissue demonstrated positive staining; (2) positive staining was also observed in all 8 rats with hepatoma and in 62.5% of hepatoma tissue; (3) in <SUP>14</SUP>C-leucine uptake studies, the incorp ration rate into the albumin fraction was 30.9% for hepatocytes from normal liver tissue and 40.1% for hepatocytes from hepatoma-bearing liver tissue; (4) tissue albumin con-centration as determined by Laurell-Rocket method was 4.2mg/wet.g. liver in hepatoma tissue and 0.98mg/wet.g. liver, in non-hepatoma tissue; and (5) the paradoxic results from the <SUP>14</SUP>C-leucine uptake experiments and the tissue albumin concentration determinations that albumin release from hepatoma cells differ from that of normal hepatocytes. <BR>In conclusion, both human and chemically-induced rat hepatoma cells perform albumin synthesis.
- 社団法人 日本肝臓学会の論文
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- ヒト肝細胞癌および実験肝癌における癌細胞の蛋白合成能に関する研究--アルブミン合成能について
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