ラット肝isoferritin phenotypeに関する実験的研究-1-鉄投与によるbasic shiftとその機序

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The mechanisms accounting for the phenotypic shift of isoferritins in haemochromatosis were investigated by experimental models using Sprague-Dawley rats. The iron was administered intraperitoneally for five consecutive days and the purified ferritin was analyzed by isoelectric focusing and SDS/polyacrylamide gel electrophoresis. Isoferritin phenotype shifted to more basic form dcpending on the increased amount of admnistered iron. In addition, the iron content of ferritin and the relative amount of L-subunits were elevated progressively with the iron dose. Pulse labelling study using <SUP>3</SUP>H-leucine also showed an increased uptake of radioactivity into ferritin protein, particu1ar into the L-subunit by iron administration. These findings suggests that this basic shift results from the increased amount of L-subunits in ferritin molccule.
社団法人日本肝臓学会の論文