THE CELLULAR BIOLOGY OF A NEWLY ESTABLISHED CELL LINE OF HUMAN OVARIAN ADENOCARCINOMA IN VITRO
スポンサーリンク
概要
- 論文の詳細を見る
Upon cultivation of various human ovarian cancer, a successful establish ment was made on a long term continuous culture of the papillary serous cystadenocarcinoma. This new cell line was named human ovarian cancer twenty-one (HOC-21).The material obtained from an ovarian cancer on the left side of a 56 year-old woman, was placed into culture on September 22, 1971. A favorable growth has been recognized from the time of culture till now, for a period of 2 years and 4 months, and presents no signs of declining. Doubling time was 28 hours at the 26th generation.The cultured cells were commonly polygonal and epithelial in shape and the nuclei revealed pleomorphism and proved clearly to be atypical under exfoliative cytology. A jigsaw puzzle like cellular arrangement, wheeled pat tern and piling-up tendency in the growing process were characteristics of the cells. And sometimes a vesicle formation was shown.The PAS staining in the cultured cell was negative; alkaline phosphatase was negative and acid phosphatase positive.Chromosomes, manifesting near-diploid, possessed a large telocentric marker chromosome.When HOC-21 was transplanted into a hamster cheek pouch, it demon strated a definite tumor formation whose histologic picture revealed papillary adenocarcinoma. The original characteristics of ovarian adenocarcinoma have been precisely maintained in vitro culture system.
- The Keio Journal of Medicineの論文
著者
-
Yamada Takuro
Department Of Emergency Medicine Gunma University Graduate School Of Medicine
-
YAMADA TAKURO
Department of Obstetrics and Gynecology, School of Medicine, Keio University
関連論文
- Prognostic Value of p53 Protein Accumulation in Cancer Cell Nuclei in Adenocarcinoma of the Uterine Cervix
- Peritoneal Washing Cytology in Small Cell Carcinoma of the Uterine Cervix : A case report
- Different expression patterns of KIT, EGFR, and HER-2 (c-erbB-2) oncoproteins between epithelial and mesenchymal components in uterine carcinosarcoma
- Echo Free Space in Morrison's Pouch in a Patient with Traumatic Bladder Rupture : A Case Report
- Usefulness of Multidetector Computed Tomography(M DCT) for the Initial Evaluation of Multiple Blunt Trauma of the Trunk
- Sub-acute Toxicosis Caused by a Multiple Doses Tegafur/Uracil (UFT) for Suicide : A Case Report
- Bilateral Internal Mammary Artery Injury without Fracture of the Chest Following a Traffic Accident
- THE CELLULAR BIOLOGY OF A NEWLY ESTABLISHED CELL LINE OF HUMAN OVARIAN ADENOCARCINOMA IN VITRO