Inhibition of Capsular Protein Synthesis of Pasteurella multocida Strain P-1059

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概要

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• A mutant strain PBA322 was constructed by electroporation of phagemid containing coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into parental strain P-1059 (serovar A:3) of Pasteurella multocida and determined the pathogenicity in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed by the obliquely-transmitted light stereomicroscope when compared to iridescent colonies of the parental strain. Moreover, strain PBA322 showed low amount of OmpH when compared to the parental strain on SDS-PAGE. Additionally, capsule thickness of strain PBA322 was thinner than parental strain by electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens.

著者

• KATAOKA Yasushi

  Laboratory of Veterinary Microbiology, Nippon Veterinary and Life Science University

• Sawada Takuo

  Laboratory Of Microbiology Faculty Of Veterinary Medicine Nippon Veterinary And Life Sciences Univer

• STHITMATEE Nattawooti

  Department of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University

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