Generation of human monoclonal immunoglobulins secreted by human B cell-murine myeloma cell heterohybridomas.
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概要
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We generated human monoclonal immunoglobulins secreted by human-mouse heterohybridomas. When EB virus transformed B lymphoblastoid cells were used as fusion partner, IgM secreting heterohybridomas were generated. After culturing cell lines for longer time, hybridization produced more clones. When pokeweed mitogen stimulated lymphoblastoid cells were used as fusion partner, IgM, IgG, and IgA secreting heterohybridomas were generated. These human monoclonal immunoglobulins library can be useful for analysis of human immunoglobulin allotypes.
- 日本臨床免疫学会の論文
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- Generation of human monoclonal immunoglobulins secreted by human B cell-murine myeloma cell heterohybridomas.