Clinical usefulness of anti double-stranded DNA antibodies detected by radioimmunoassay with enlarged measuring scale.

概要

論文の詳細を見る
Farr's assay using 125I labelled double-stranded DNA (dsDNA) obtained from E. coli plasmid by recombinant technique is the most sensitive and specific method for the detection of anti ds-DNA antibodies in patients with clinically active systemic lupus erythematosus (SLE). Because of insufficient dsDNA antigen, however, final antibody levels were hardly determined when the sera contained antibodies more than 100U/ml. A new Farr's assay has been developed by increasing dsDNA antigen, which enlarged calibration range from 0100U/ml up to 0300IU/ml. The final antibody levels of thirty two out of thirty nine SLE sera (82.1%) with antibodies more than 100U/ml by current Farr's assay have been measurable by the new Farr's assay. An excellent correlation has been observed between two assays in sera showing antibody less than 100U/ml by current Farr's assay (r=0.9346, p<0.01).The application of newly developed Farr's assay, thus, is a useful tool for the evaluation of clinical characteristics and course of SLE patients who had high levels of anti dsDNA antibodies.
日本臨床免疫学会の論文