Cell surface phenotypes of B cells producing class-specific anti-DNA antibodies: Correlation with Ly-1 B cells.

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We investigated cell surface phenotypes of anti-DNA antibody-producing B cells from NZB×NZW (B/W) F 1 mice, using several monoclonal antibodies (mAb) to B cells includ. ing Ly-1 (CD 5) and our newly established Lp-3 which defines a unique B cell differentiation antigen. The 2-mo-old B/W F 1 mice spontaneously produced IgM-class but not IgG-class anti-DNA antibodies, while the 7- to 8-mo-old B/W F 1 mice produced mainly IgG and less amounts of IgM anti-DNA antibodies. In in vitro studies using FACS-sorted B cells, we found that surface phenotypes of the IgM anti-DNA antibody-producing B cells are Ly-1+, Lp-3-, sIgM+ and CD 45 R+, whereas those of IgG antibody-producing B cells are Ly-1-, Lp-3+, sIgM-, sIgG+ and CD45R+. The majority of peritoneal B cells from either 2- or 7-mo-old B/ W F 1 mice was Ly-1+, Lp-3+, sIgM+ and CD45R+, and produced neither, if at all, IgM not IgG anti-DNA antibodies. Whether such phenotypical distinction represents the differences in the lineage or the changes during differentiation of B cells is discussed.
日本臨床免疫学会の論文