Heterogeneity of LAK cells by investigation of class I and II antigen on target cells.

概要

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A possible involvement of major histocompatibility complex (MHC) antigens in lysis of target cells by autologous lymphokine-activated killer (LAK) cells was studied in human systems. LAK cells that were generated by 7-day culture of peripheral blood lymphocytes with recombinant interleukin 2 (IL 2) killed autologous phytohemagglutinin (PHA)-induced T blasts in a 4-h 51Cr release assay. NK type LAK cells expressing CD 16 antigen showed higher cytotoxicity against autologous T blasts that were depleted of MHC class I antigens by citric acid treatment, with little reaction recorded against MHC class I positive targets. By contrast, CTL type LAK cells with CD3 antigens on their surface preferentially killed autologous T blasts that were induced to express higher amounts of MHC class I antigens by recombinant interferon-gamma treatment. On the other hand, class II antigens were not involved in LAK-mediated lysis of autologous T blasts. In kinetics the NK type LAK cells appeared on day 3 of IL 2 treatment, the activity of which peaked on day 5 and then declined on day 7. By contrast, the CTL type LAK activity was noticeable after 5 day, reaching a maximum on day 7. These results indicate that LAK cells could be classified according to their ability to kill MHC class I positive and negative autologous target cells.
日本臨床免疫学会の論文