Detection of antibody for HTLV-I by Behring ELISA Processor II and clinical examination about HTLV-I carriers

スポンサーリンク

概要

• 論文の詳細を見る

• A rapid and automated method of an enzyme linked immunosorbent assay (EIA) was devised for the detection of antibodies to adult T-cell leukemia associated antigens (ATLA, HTLV-I), by the combined use of HTLV-I coated microcup E-0773 (Eisai) and Behring ELISA Processor II (Hoechst). Comparing to the ordinary method of EIA using the Corty series (Toshiba Co. Ltd.), the results were correlated well with "Five times" of washing program.The absorbance of twenty sera of SLE, ten sera of ATL and ten normal sera were elevated variously by the inactivation with heat treatment (56°C, 30min.), however, only ATL sera were inhibited specifically by the absorption test with virus antigen. Furthermore, eighteen out of 1354 sera which were inactivated by heat treatment were also inhibited specifically with the inhibition rate over 1.0 O.D. in all of them.HTLV-I carriers were 1.6% of all patients examined and were detected highly in Parkinsonism, polyneuritis, hepatoma, esophageal cancer, polyarthritis, and blood diseases. An appearance of atypical lymphocytes (50%), and myelocytes (48%) in the peripheral blood were remarkable in these carriers. By the Western blot method P-40 were detected in five cases.

• 日本臨床免疫学会の論文

著者

• 石森 章

  東北大学臨床検査診断学

• 菅村 和夫

  東北大学細菌

• 本田 一陽

  東北大学臨床検査診断学

• 渡辺 正子

  東北大学臨床検査診断学

• 戸田 順子

  東北大学臨床検査診断学

• 永田 欣也

  東北大学細菌学

関連論文

• 18. 胃幽門洞切除後の迷走神経刺激による門脈血中消化管ホルモン(第16回迷切研究会)
• 33 迷走神経刺激時の消化管ホルモン分泌に及ぼす胃幽門洞切除術の影響(第30回日本消化器外科学会総会)
• 3. ヒトパルボウイスル B19 感染とリウマチ性関節炎 (VII 感染と免疫異常の接点)
• 2.Polymerase chain reaction 法による胆道閉鎖症のレオ3型ウイルス検索(第17回胆道閉鎖症研究会)
• Clinical test.2.Detailed explanations.The new inspections which should be known by an internist.3.Digestive tract diseases.
• Detection of antibody for HTLV-I by Behring ELISA Processor II and clinical examination about HTLV-I carriers

スポンサーリンク