Effect of calcium channel blocker on human natural killer activity

概要

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In this study, the in vitro effect of niphedipine as a calcium channel blocker on human natural killer (NK) activity was examined. NK activity against K562 cells was inhibited depending on the dosage of niphedipine added in vitro (it was remarkably inhibited at the concentration of 3.6 × 103 ng/ml), but it was not inhibited after preincubation of effector cells with niphedipine. Kinetic studies of the effect of niphedipine on NK activity showed that niphedipine depressed the early events of NK cytolysis.In vitro adding of interferon-α (IFN-α) restored the NK activity depression by niphedipine, and the augmentation of NK activity after preincubation of effector cells with IFN-α was not inhibited by niphedipine.These results indicate that calcium ions play an important role in the mechanisms of NK cytolysis but suggest that augmentation of NK activity by IFN-α in a part of this mechanism may be developed without Cat2+ as a second messenger.
日本臨床免疫学会の論文