Augmentation of K-cell activity by human interferon : Analysis of the mechanism at a single cell level using plaque assay method

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In order to resolve the question whether interferon (IF)-induced augmentation of human ADCC activity is due to an increase of the number of effector cell (K-cell) or an activation of individual effector cell, the effect of interferon on the number and size of K-cell plaque was examined at a single cell level by plaque assay method using Cunningham's chamber.In the absence of IF, the number of K-cell plaque was reached to the maximum at 8hr, whereas in the presence of IF it was more increased depending on the concentration of IF at the early time of culture (3-5hr). However, 8hr of incubation, the maximum number of K-cell plaque in the presence of IF was equal to that in the abscence of IF. Furthermore, the area of individual plaque of K-cell in the presence or abscence of IF was measured at 8hr of culture and compared with each others. It was revealed that the average area of K-cell plaque in the presence of IF was significantly larger than that in the abscence of IF.We concluded that the treatment of human peripheral lymphocytes with IF resulted in the augmentation of activity of the individual K-cell, but not the increase of number of K-cell.