脳血栓患者における血小板凝集能測定について

概要

論文の詳細を見る
We have previously cloned and characterized both bovine male-specific and genderneutral DNAs. Primers for the polymerase chain reaction (PCR) were synthesized on the basis of these DNAs to give differential PCR products between males and females. Serial dilution of bovine somatic cells of both sexes revealed that 10 cells were sufficient to discriminate males from females under our conditions. The usefulness of our primers to sex bovine embryos was confirmed by using purified control DNAs, cytogenetic analysis of demi-embryos, coincidence of a pair of asymmetrical biopsy samples and embryo transfer. All the embryos used here were produced by <I>in vitro</I> fertilization and culture methods. A successful determination of sex was made for 83 out of 84 embryos examined (98.8%). The sex ratio (54.2% male) did not differ significantly from the expected 1:1 ratio. The viability of biopsied embryos (86.3%) after 24 h culture <I>in vitro</I> was not significantly different from that of intact embryos (92.3%). Also, the mean numbers of cells of the biopsied (153.1, n=42) and intact (170.7, n=39) embryos were not significantly different. Out of seven recipients received the embryos with predicted sex, three went to term, producing one male and 2 female calves in accordance with the sex prediction before transfer. In conclusion, our method appears to be an efficient and reliable one to determine the sex of bovine preimplantation embryos.