Effects of Prolonged Sperm Preincubation and Elevated Calcium Concentration on Fertilization of Cumulus-Free Mouse Eggs <I>in Vitro</I>

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概要

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• Freshly superovulated eggs of Jcl: ICR mature mice that had been freed from their cumulus oophorus with hyaluronidase were fertilized by epididymal spermatozoa preincubated for various periods in a modified Krebs-Ringer bicarbonate medium (TYH). Calcium concentration in the fertilization medium was elevated to the twice (3.42 mM) or to the 3 times (5.13 mM) of TYH (1.71 mM) and all incubations were performed at 37C under 5% CO<SUB>2</SUB> in air. Both the sperm preincubation time and the concentration of calcium in the fertilization medium were found to exert significant effects on fertilization of cumulus-free eggs <I>in vitro</I>, and the highest fertilization rate was obtained when the cumulus-free eggs were inseminated with 150 min-preincubated spermatozoa in a medium with 3.42 mM calcium.

• 日本繁殖生物学会の論文

著者

• ITAGAKI Yoshiaki

  Central Research Institute, Itoham Foods Inc.

• TOYODA Yutaka

  Institute of Medical Science, University of Tokyo

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• 脳血栓患者における血小板凝集能測定について
• Effects of Prolonged Sperm Preincubation and Elevated Calcium Concentration on Fertilization of Cumulus-Free Mouse Eggs in Vitro
• Differences in Early Cleavage Rates of In Vitro Produced Bovine Embryos Classified According to the Completion of Their First Cleavage

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