Studies of Mammalian Implantation <I>In Vitro</I> (II): Effects of Cytokines from Mixed Lymphocyte Reaction on Proliferation of Murine Trophoblast Cell and Establishment of Its Cell Line
スポンサーリンク
概要
- 論文の詳細を見る
We report here that the supernates of mixed lymphocyte reaction (MLR) enhanced the proliferation of trophoblast cells. The analysis of the factors in MLR supernates revealed that a series of CSF family cytokines such as IL-3/GM-CSF and M-CSF, which seemed to be responsible for stimulation of the proliferation of trophoblast cells, were significantly raised in MLR supernates. At the same time, the effects of recombinant human (rh) IL-3/GM-CSF and M-CSF on the proliferation of the trophoblast cells were investigated. The rhlL-3/GM-CSF gave slight proliferation-promoting effects while the rhM-CSF showed stronger stimulating effect which was comparable to the MLR supernates. Continuous addition of the MLR supernates into the primary culture of trophoblast cells resulted in the establishment of trphoblast cell line. The cells showed the polygonal epithelium like appearances and reacted positively with anti-cytokeratin antibodies but not with anti-vimentin antibodies by means of indirect immunofluorescence (IIF) test.
- 日本繁殖生物学会の論文
著者
-
Mori Tsuneatsu
Department Of Immunology Institute Of Medical Science University Of Tokyo
-
KIKKAWA Yukio
Department of Immunology, Institute of Medical Science, University of Tokyo
-
NOMURA Ko
Department of Immunology, Institute of Medical Science, University of Tokyo
-
KANAYA Mika
Department of Gynecology and Obstetrics, Sapporo Medical College
-
TANAKA Shoichi
Department of Gynecology and Obstetrics, Sapporo Medical College
-
OHASHI Hideya
Kirin Brewery Co., Ltd. Pharmaceutical Laboratory
-
SUDO Tadasi
Kirin Brewery Co., Ltd. Pharmaceutical Laboratory
-
Mori Tsuneatsu
Department Of Biochemistry Institute Of Medical Science University Of Tokyo
関連論文
- Human T Cell Leukemia Cell Death by Apoptosis-inducing Nucleosides from CD57^+ HLA-DR^ Natural Suppressor Cell Line
- Molecular Mechanism in Mammalian Fertilization
- Purification of N-acetyllactosamine-binding Activity from the Porcine Sperm Membrane: Possible Involvement of an ADAM Complex in the Carbohydrate-binding Activity of Sperm
- Studies of Mammalian Implantation In Vitro (I): Production of Laminin from Human Decidual Cell Line and Its Effects on Murine Trophoblastic Outgrowth
- Studies of Mammalian Implantation In Vitro (II): Effects of Cytokines from Mixed Lymphocyte Reaction on Proliferation of Murine Trophoblast Cell and Establishment of Its Cell Line
- Purification of N-acetyllactosamine-binding Activity from the Porcine Sperm Membrane : Possible Involvement of an ADAM Complex in the Carbohydrate-binding Activity of Sperm