Production of Prostaglandin F<SUB>2α</SUB> (PGF<SUB>2α</SUB>) in Primary Culture of Granulosa Cells from Mature Follicles of Estrous Cows

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概要

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• Granulosa cells(GC) collected from follicular fluids of the mature follicles of 9 estrous cows were cultured for 6, 12 or 24 h at 37C in a serum-free synthetic culture medium (SMF-101 "Nissui"). On completion of the culture periods, the concentrations of PGF<SUB>2α</SUB>, in the culture supernatants were determined by radioimmunoassay. The following results were obtained. The PCF<SUB>2α</SUB> concentrations in the culture supernatants were increased in 8 of the 9 samples as the culture elapsed. Statistic analysis was carried out by Tukeys q-test. Significant differences were found in the PGF<SUB>2α</SUB>, concentrations in the supernatants between 6 and 24h culture. This suggested that GC derived from the mature follicles of the estrous stage of cows produced PGF<SUB>2α</SUB>, in the medium.

• 日本繁殖生物学会の論文

著者

• Kawakami Shizuo

  Department Of Theriogenology Azabu University

• KOHMOTO Hidemichi

  Futtu Branch Office, Central Animal Clinical Center, Chiba Prefectural Federation of Agricultural Mu

• KOHMOTO Hidemichi

  Futtu Branch Office, Central Animal Clinical Center, Chiba Prefectural Federation of Agricultural Mutual Relief Associations

• KAWAKAMI Shizuo

  Department of Theriogenology, School of Veterinary Medicine, Azabu University

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• Production of Prostaglandin F2α (PGF2α) in Primary Culture of Granulosa Cells from Mature Follicles of Estrous Cows

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