Measurement of Oxygen-Derived Free Radical Generation in the Regionally-Ischemic Rat Heart by the Chemiluminescence Method.

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概要

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• Generation of oxygen-derived free radicals (oxy-radicals) in the stored rat heart was measured by chemiluminescence. Hearts subjected (ischemia) or not subjected (non-ischemia) to a 4-min regional ischemia were frozen in liquid nitrogen and stored until assayed. The frozen myocardium was ground and oxygenated by mixing it with phosphate-buffered saline (Po<SUB>2</SUB>: 194 mmHg) containing lucigenin. The chemiluminescence intensity of ischemic myocardium was larger than that of non-ischemic myocardium. Recombinant human superoxide dismutase significantly decreased these intensities. These results indicate that O<SUB>2</SUB><SUP>-</SUP> is one of the major oxy-radical species in the rat heart and that the generation of oxyradicals is enhanced by regional ischemia for 4 min.

• 公益社団法人 日本薬理学会の論文

著者

• Ohoi Itaru

  Pharmacology Section Laboratory Of Biology Research Laboratories Pharmacological Group Nippon Kayaku

• Nakamura Keita

  Pharmacology Section, Research Laboratories, Nippon Kayaku Co., Ltd.

• Tobari Hiroko

  Pharmacology Section, Research Laboratories, Nippon Kayaku Co., Ltd.

関連論文

• Effects of a Novel Cyclohexane Dicarboximide Derivative, ST-6, on Reperfusion-Induced Arrhythmia in Rats
• A Simple Chemiluminescence Method for Measuring Oxygen-Derived Free Radicals Generated in Oxygenated Rat Myocardium.
• Measurement of Oxygen-Derived Free Radical Generation in the Regionally-Ischemic Rat Heart by the Chemiluminescence Method.

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