Bovine brain coated vesicles contain guanine nucleotide regulatory proteins.

スポンサーリンク

概要

• 論文の詳細を見る

• Binding of [<SUP>3</SUP>H]guanosine triphosphate (GTP) with a high affinity was found to be present in the coated vesicle fraction prepared from bovine cerebral cortex. The binding was saturable and displaced by 1μM of GTP, guanosine diphosphate and guanosine 5'-(3-O-thio)triphosphate. Incubation of the vesicles with islet-activating protein and [<SUP>32</SUP>P]NAD resulted in ADP-ribosylation of a 39, 000—41, 000-dalton polypeptide. Antibodies to the α-subunit of stimulatory guanine nucleotide regulatory proteins (G-proteins) immunoblotted 52, 000 and 45, 000-dalton polypeptides. The results indicate that stimulatory and inhibitory G-proteins are contained in a fraction of the bovine brain coated vesicles.

• 公益社団法人 日本薬理学会の論文

著者

• MOROI Kayoko

  Division of Cardiovascular Biology, Center for Biomedical Science, Chiba University School of Medici

• KUGA Tetsuro

  Division of Functional Neurobiology, Center for Neurobiology and Molecular Immunology, School of Medicine, Chiba University

• Kadota Ken

  Department of Neurochemistry, Psychiatric Research Institute of Tokyo

関連論文

• Two Different Endothelin B Receptor Subtypes Mediate Contraction of the Rabbit Saphenous vein
• Endothelin ET_A and ET_B Receptors Mediate Endothelin-1-Induced Apamin-Sensitive Relaxation in the Guinea Pig Ileum
• P BIOASSAY OF ACETYLCHOLINE WITH A THIN MUSCLE STRIP OF THE JAPANESE MEDICAL LEECH: A PRELIMINARY NOTE ON THE REAPPRAISAL OF THE AVAILABILITY OF THIS METHOD
• SPECIFICITY OF MUSCLE RESPONSE OF THE JAPANESE MEDICAL LEECH TO ACETYLCHOLINE
• Detection and characterization of 3H-clonidine binding sites in coated vesicles isolated from bovine brain.
• Bovine brain coated vesicles contain guanine nucleotide regulatory proteins.

スポンサーリンク