Detection and characterization of 3H-clonidine binding sites in coated vesicles isolated from bovine brain.

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概要

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• The binding of <SUP>3</SUP>H-clonidine to bovine brain coated vesicles was studied. The binding was reversible and saturable. Saturation studies revealed a one-site interaction: K<SUB>d</SUB> was 8.7 nM and B<SUB>max</SUB> was 24.5 fmol/mg protein. Noradrenaline, yohimbine and phenoxybenzamine displaced <SUP>3</SUP>H-clonidine binding from the binding sites at concentrations of 10<SUP>-7</SUP>-10<SUP>-4</SUP> M. The results indicate that the coated vesicles from the bovine cerebral cortex contain <SUP>3</SUP>H-clonidine binding sites comparable to the low affinity sites of α<SUB>2</SUB>-adrenergic receptors in bovine brain membranes.

• 公益社団法人 日本薬理学会の論文

著者

• MOROI Kayoko

  Division of Cardiovascular Biology, Center for Biomedical Science, Chiba University School of Medici

• KADOTA Ken

  Department of Pharmacology, Osaka University Medical School

• Moroi Kayoko

  Division of Functional Neurobiology, Center for Neurobiology and Molecular Immunology, School of Medicine, Chiba University

• KUGA Tetsuro

  Division of Functional Neurobiology, Center for Neurobiology and Molecular Immunology, School of Medicine, Chiba University

• Kadota Ken

  Department of Neurochemistry, Psychiatric Research Institute of Tokyo

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• Detection and characterization of 3H-clonidine binding sites in coated vesicles isolated from bovine brain.
• Bovine brain coated vesicles contain guanine nucleotide regulatory proteins.

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