カナマイシン聾モルモットのラセン器内陰性電位の測定

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The negative DC resting potential inside the organ of Corti of the guinea pig with normal hearing and the kanamycin-poisoned guinea pig was measured. The level of the potential was about -80 mV both in normal and kanamycin-poisoned guinea pig.In the normal guinea pig, the potential decreased gradually when the tip of electrode was attached to the fixed point of the organ of Corti for several minutes, and the potential of similar level was derived again when electrode was pushed slightly deeper. This indicates that the electrode records the intracellular potential, and then damages the cell so that its negativity is decreased. When the electrode is pushed slightly deeper, the electrode enters another cell and records the potential again.In the kanamycin-poisoned guinea pig, the potential decreased gradually when the tip of electrode was attached to the fixed point of the organ of Corti for several minutes, but the potential was not recorded again although electrode was pushed deeper. The histological examination of the cochlea of the kanamycin-poisoned guinea pig after this experiment revealed that the electrode penetrated the extracellular space in the organ of Corti where no cellular elements were recognized due to the degeneration of the outer hair cells.It is generally accepted that, in normal condition, the negativity inside the organ of Corti is intracellular in origin, and the hypothesis that this negativity is recorded from the fluid surrounding the hair cells seems to be denied by many investigators. However, the possibility that this fluid of the kanamycin-poisoned guiaea pig is negatively polarized because of the electrical leakage from the degenerated outer hair cells and thus the dendrites of the 8th nerve which pass through the fluid are depolarized could be considered from the observations of this experiment. This will explain the origin of the deafness induced by kanamycin-poisoning.
社団法人日本耳鼻咽喉科学会の論文

カナマイシン聾モルモットのラセン器内陰性電位の測定

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