Quantitation of L-Arginine and Asymmetric Dimethylarginine in Human Plasma by LC-Selective Ion Mode-MS for Type 2 Diabetes Mellitus Study

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概要

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• The article reports a simple, sensitive and fast LC/MS method for the analysis of L-arginine (L-Arg), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) in human plasma. The homoarginine was used as the internal standard (IS). The chromatographic separation was achieved on C18 (150 mm×2.1 mm, 5 μm) column with a mobile phase consisting of ammonium acetate (0.25 mmol/l) and methanol (93 : 7, v/v), at a flow rate of 0.2 ml/min. L-Arg, ADMA and SDMA were well separated by LC/MS with selective ion mode (SIM). The method was successfully applied to type 2 diabetes mellitus (T2DM) study. Twenty-one healthy controls and twenty-two T2DM patients before and after treatment two years were investigated. The results indicated that the level of ADMA in T2DM was significantly higher than that in healthy controls. Furthermore, ADMA has important association with the development of cardiovascular diseases.

著者

• Liang Yizeng

  Research Center For Modernization Of Chinese Herbal Medicine College Of Chemistry And Chemical Engin

• Yi Lunzhao

  Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University

• Zhang Pengfei

  Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University

• Ji Xiaoyan

  Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University

• Wang Yamin

  Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University

• Zhou Zhiguang

  Diabetes Center, The Second Xiangya Hospital, Central South University

• Chen Benmei

  Analytical Testing Center of School of Xiangya Medicine, Central South University

関連論文

• Quantitation of L-Arginine and Asymmetric Dimethylarginine in Human Plasma by LC-Selective Ion Mode-MS for Type 2 Diabetes Mellitus Study
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