反芻動物の蛋白質消化に関する研究-1-
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概要
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Casein degradation in the rumen was studied with a fistulated sheep both in vivo and in vitro. The results obtained are as follows.1. Protein-N concentration in the rumen fluid increased rapidly with the lapse of time after feeding, attaining a maximum in 2 hours after, and then decreased until 7 hours. Amino-N concentration also increased up to 2 hours and then decrease gradually. Ammonia-N concentration, however, continued to increase and exceeded the other two nitrogen fractions after 5 hours. From these results, it was suggested that casein taken in by an animal was degraded to peptides and amino acids, from which ammonia was liberated secondly in the rumen.2. An experiment was carried out in vitro with washed suspension of the rumen bacteria, which had been obtained from a sheep on a casein ration. Amino-N was produced from casein, but no ammonia production occurred under the conditions employed. Therefore, it is apparent that degradation of protein to peptides and amino acids largely depends on the bacterial action in the rumen. Further investigation may necessary to discuss the biochemical nature of ammonia formation in the rumen.
- 社団法人 日本畜産学会の論文