鶏の下垂体前葉の細胞化学的研究 : III 副腎除去及びcorticosteroid投与の影響

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Fourteen white Leghorn cockerels, 3 to 4 months of age, were adrenalectomized and sacrificed at different times for 12 hours to 8 days after adrenalectomy. Of these 2 birds were injected with desoxycorticosterone-acetate (DOCA, 5mg per day) for 4 days and sacrificed 5 days after the operation Two unilaterally adrenalectomized, 8 cortisone-tre-ated and 6 untreated cockerels of the same age were used as the controls. For the purpose of cyto-logical studies, the pituitaries of these birds were fixed in formol-sublimate and stained by GOLDERG and CHAIKOFFS method (52), HETDENHAINS azan te-chnique, GOMORIS A-F method, PAS method and metachromatic staining with many basic dyes. The results obtained are as follows: (1) No alteration was shown in the number and the cytological structure of the α cells in the caudal lobe following adrenalectomy or injection with corticosteroid. This suggests that the α cells have no relation with the secretion of adrenocorticatrophin (ACTH).(2) The adrenalectomized birds showed such remarkable changes as disappearance of granulation, pyknosis of the nuclei, and reduction in number and size of the β ce1s and were stained quite feebly.Degenerative cells commonly refered to as CROOKEe cells appeared abundantly in the caudal lobe of the adrenalectomized or cortisone-treated birds. The βcells of the adrenalectomized birds were not completely recovered from these degenerative changes by the injection of DOCA. Accordingly, the cy tological picture of the βcells did dot seem to correlate to an increase or decrease in demand for endogenous ACTH.(3) The most remarkable change in the cytological picture of the pituitary following adrenalectomy was observed in the V cells of the cephalic lobe. They were increased distinctly in number, enlarged to their maximum size, and showed a chromophobe appearance with little granulation in cytoplasm. Their uclei were markedly enlarged containing two or three(or occasionally more) clear, spherical nucleoli each, which became larger in accordance with the growth of the cells. The injection of DOCA prevented any changes following adrenalectomy in the V cells of the pituitary. These observations made on the cytological changes in the pituitary indicate that only the V cells were associated with the ACTH secretion following adrenalectomy or treatment with corticosteroid. The PAS-positive granule of the V cells is not a chemical component of ACTH at all, but it may be cement or a carrier substance of ACTH.
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鶏の下垂体前葉の細胞化学的研究 : III 副腎除去及びcorticosteroid投与の影響

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