Analysis of Bacterial Populations in the Environment Using Two-dimensional Gel Electrophoresis of Genomic DNA and Complementary DNA

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概要

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• Two-dimensional gel electrophoresis (2-DGE) mapping of genomic DNA and complementary DNA (cDNA) amplicons was attempted to analyze total and active bacterial populations within soil and activated sludge samples. Distinct differences in the number and species of bacterial populations and those that were metabolically active at the time of sampling were visually observed especially for the soil community. Statistical analyses and sequencing based on the 2-DGE data further revealed the relationships between total and active bacterial populations within each community. This high-resolution technique would be useful for obtaining a better understanding of bacterial population structures in the environment.

• 日本微生物生態学会・日本土壌微生物学会の論文

著者

• ITOH Kiminori

  Graduate School of Environment and Information Sciences, Yokohama National University

• RAJENDRAN NARASIMMALU

  Graduate School of Environment and Information Sciences, Yokohama National University

• AMEMIYA TAKASHI

  Graduate School of Environment and Information Sciences, Yokohama National University

• Rajendran Narasimmalu

  Graduate School Of Engineering Yokohama National University

• Liu Guo-hua

  Graduate School Of Engineering Yokohama National University

• Itoh Kiminori

  Graduate School Of Engineering Yokohama National Univ. 79-7 Tokiwadai Hodogaya-ku Yokohama 240-8501

• Nakamura Tatsuo

  Graduate School of Environment and Information Sciences, Yokohama National University

• Amemiya Takashi

  Graduate School Of Environment And Information Sciences Yokohama National University

• Amemiya Takashi

  Graduate School Of Environment And Information Science Yokohama National University

• Nakamura Tatsuo

  Graduate School Of Environment And Information Sciences Yokohama National University

• Itoh Kiminori

  Graduate School Of Engineering Yokohama National Univ.

• Liu Guo-Hua

  Graduate School of Engineering, Yokohama National University

• Rajendran Narasimmalu

  Graduate School of Engineering, Yokohama National University

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