Preventive and Therapeutic Effects Ramulus Mori Extract on Collagen-Induced Arthritis in Mice via Suppression of Inflammatory T Cells

スポンサーリンク

概要

• 論文の詳細を見る

• We researched the preventive and therapeutic activities of the extract of Ramulus Mori (ERM) to observe its effects on collagen-induced arthritis (CIA) in mice and to explore the mechanisms of ERM in the treatment of rheumatoid arthritis (RA). We examined the in vitro levels of tumor necrosis factor α (TNF-α) released in macrophages and of interferon (IFN)-γ and interleukin (IL)-4 released in splenocytes. For in vivo experiments, we randomly divided 24 mice into four groups, after type II collagen (CII) injections and at euthanization. The levels of plasma cytokines (TNF-α, IL-6, IL-17), rheumatoid factor (RF; IgG, IgM), and anti-CII antibody were measured using ELISA kits. The number of immunocytes (CD4+ T cells, CD3+/CD69+ T cells, B220+/CD45+ B cells, CD11b+/Gr-1+ cells) relative to RA was calculated using flow cytometry (FACS). The articular index (AI) was recorded once a week for 4 weeks. Sections of tissues from the knee joints were stained with hematoxylin and eosin (H&E) and Masson trichrome (MT) after the mice with CIA were euthanized. ERM reduced the levels of TNF-α in macrophages and IFN-γ in spleen cells, decreased AI scores, and improved inflammation of paw joints (PJ). ERM also suppressed the number of immunocytes in peripheral blood mononuclear cells (PBMCs) and PJ, and reduced the levels of cytokines (TNF-α, IL-6, and IL-17), RF, and anti-CII antibody in the sera. TNF-α and Th1 cells play very important roles in the formation and development of RA. ERM and methotrexate notably decreased the induction of RA in the CIA mice model by reducing the levels of inflammatory cytokines and RF in the sera and suppressing the number of immunocytes among PBMCs and PJ.

著者

• Roh Seong-Soo

  Department of Preparatory Oriental Medicine, College of Oriental Medicine, Daegu Haany University

• Kim Yong-ung

  Department of Herbal Pharmaceutical Engineering, College of Herbal Bio-industry, Daegu Haany Univers

• SEO Young-Bae

  Department of Oriental Medicine, Daejeon University

• Seo Young-bae

  Department Of Oriental Medicine Daejeon University

• Seo Bu-Il

  Department of Herbology, College of Oriental Medicine, Daegu Haany University

• Ku Sae-Kwang

  Department of Histology and Anatomy, College of Oriental Medicine, Daegu Haany University

• Kim Yong-ung

  Department Of Herbal Pharmaceutical Engineering College Of Herbal Bio-industry Daegu Haany Universit

• Ku Sae-kwang

  Department Of Anatomy And Histology College Of Oriental Medicine Daegu Haany University

• Seo Young-Bae

  Department of Herbology, College of Oriental Medicine, Daejeon University

• Roh Seong-Soo

  Department of Herbology, College of Oriental Medicine, Daegu Haany University

• Kim Yong-Ung

  Department of Korean Internal Medicine, College of Oriental Medicine, Daegu Haany University

関連論文

• Abietic Acid from Resina Pini of Pinus Species as a Testosterone 5α-Reductase Inhibitor
• Shengmai-san-Mediated Enhancement of Regenerative Responses of Spinal Cord Axons After Injury in Rats
• Effects of Magnetic Infrared Laser on Xylene-induced Acute Inflammation in Mice
• Preventive and Therapeutic Effects Ramulus Mori Extract on Collagen-Induced Arthritis in Mice via Suppression of Inflammatory T Cells
• Antidermatophyte and antimelanogenesis compound from Eleutherine americana grown in Indonesia
• Effects of Magnetic Infrared Laser Irradiation on Formalin-Induced Chronic Paw Inflammation of Mice
• Effects of Magnetic Infrared Laser on Dinitrofluorobenzene-induced Contact Dermatitis in Mice
• Antioxidative compounds from leaves of Tahongai (Klienhovia hospita)
• Antioxidative compounds from leaves of Tahongai (Klienhovia hospita)

スポンサーリンク