Natural Killer Group 2A (NKG2A) and Natural Killer Group 2C (NKG2C) Bind to Sulfated Glycans and α2,3-NeuAc-containing Glycoproteins

スポンサーリンク

概要

• 論文の詳細を見る

• Killer lectin-like receptors on natural killer (NK) cells mediate cytotoxicity through glycans on target cells. We prepared recombinant glutathione S-transferase-fused extracellular lectin-like domains (AA 94-231) of natural killer group 2A (NKG2A) (rGST-NKG2A) and NKG2C (rGST-NKG2C) and determined the binding of these receptors to plates coated with heparin-conjugated bovine serum albumin (heparin-BSA) and glycoproteins. rGST-NKG2A and rGST-NKG2C directly bound to heparin-BSA with Kd values of 20 and 40 nM, respectively. Binding of rGST-NKG2A and rGST-NKG2C to heparin-BSA was suppressed in the presence of soluble heparin, heparan sulfate, fucoidan, λ-carrageenan, and dextran sulfate. 2-O-Sulfate residues in heparin were essential for the binding of rGST-NKG2A and rGST-NKG2C. Moreover, rGST-NKG2A and rGST-NKG2C bound to multimeric sialyl Lewis X expressing transferrin secreted by HepG2 cells with Kd values of 80 and 114 nM, respectively. This is the first report showing that NKG2A and NKG2C bind to heparin and α2,3-NeuAc-containing glycoproteins.

著者

• ITO Kenichiro

  Department of Applied Physics, Faculty of Science and Engineering Waseda University

• Ito Kenichiro

  Department Of Applied Physics Faculty Of Science And Engineering Waseda University

• HIGAI Koji

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• MATSUMOTO Kojiro

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• Higai Koji

  Department Of Clinical Chemistry School Of Pharmaceutical Sciences Toho University

• SUZUKI CHIHO

  Department of Orthopaedic Surgery, School of Medicine, Chiba University

• Suzuki Chiho

  Department Of Orthopaedic Surgery School Of Medicine Chiba University

• Xin Xin

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• Imaizumi Yuzo

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• Itoh Ayumi

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• Matsumoto Sayo

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• Azuma Yutaro

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

• Azuma Yutaro

  Department Of Biochemistry Tokyo Dental College

• Matsumoto Kojiro

  Department Of Clinical Chemistry Faculty Of Pharmaceutical Sciences Toho University

• Xin Xin

  Dep. Of Clinical Chemistry Fac. Of Pharmaceutical Sciences Toho Univ.

• Suzuki Chiho

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Toho University

関連論文

• Clustering Motions in N-Body Systems : Computer Experiments of Kinetic Laws
• Binding Affinities of NKG2D and CD94 to Sialyl Lewis X-Expressing N-Glycans and Heparin
• Glycated Human Serum Albumin Induces Interleukin 8 mRNA Expression through Reactive Oxygen Species and NADPH Oxidase-Dependent Pathway in Monocyte-Derived U937 Cells(Biochemistry)
• Natural Killer Group 2A (NKG2A) and Natural Killer Group 2C (NKG2C) Bind to Sulfated Glycans and α2,3-NeuAc-containing Glycoproteins
• Protein O-N-Acetylglucosaminylation Modulates Promoter Activities of Cyclic AMP Response Element and Activator Protein 1 and Enhances E-Selectin Expression on HuH-7 Human Hepatoma Cells(Molecular and Cell Biology)
• Enhanced Expression of Membrane-Associated Sialidase Neu3 Decreases GD3 and Increases GM3 on the Surface of Jurkat Cells during Etoposide-Induced Apoptosis(Molecular and Cell Biology)
• Increased Expression of Lewis X and Y Antigens on the Cell Surface and FUT 4 mRNA during Granzyme B-Induced Jurkat Cell Apoptosis(Molecular and Cell Biology)
• NF кB-p65 Dependent Transcriptional Regulation of Glycosyltransferases in Human Colon Adenocarcinoma HT-29 by Stimulation with Tumor Necrosis Factor α(Highlighted paper selected by Editor-in-chief,Biochemistry)
• Targeted Proteo-Glycomics Analysis of Sialyl Lewis X Antigen Expressing Glycoproteins Secreted by Human Hepatoma Cell Line
• A Fluorometric Assay for Glycosyltransferase Activities Using Sugars Aminated and Tagged with 7-Hydroxycoumarin-3-carboxylic Acid as Substrates and High Performance Liquid Chromatography
• Repair of cartilage defects and torn acetabular labrum in hip joints after conventional osteotomy : evaluation by follow-up arthroscopy
• Effects of Protein Tyrosine Kinase Inhibitors with Different Modes of Action on Topoisomerase Activity and Death of IL-2-Dependent CTLL-2 Cells
• Nitric Oxide and Immune Complexes Are Involved in the Induction of a Novel Luminol Chemiluminescencwe in Cytotoxic Macrophages
• Human Mannose-Binding Lectin Preferentially Binds to Human Colon Adenocarcinoma Cell Lines Expressing High Amount of Lewis A and Lewis B Antigens
• Alterations in Cell Surface Phosphatidylserine and Sugar Chains during Apoptosis and Their Time-Dependent Role in Phagocytosis by Macrophages(Biochemistry/Molecular Biology)
• Unlike Natural Killer (NK) p30, Natural Cytotoxicity Receptor NKp44 Binds to Multimeric α2,3-NeuNAc-Containing N-Glycans
• Clustering Motions in N-Body Systems : Computer Experiments of Kinetic Laws
• Decreases in CD31 and CD47 Levels on the Cell Surface during Etoposide-Induced Jurkat Cell Apoptosis
• Retrograde Radiographic Development in Pulmonary Sarcoidosis
• Pleuroperitoneal Shunt for Refractory Chylothorax Accompanied with a Mediastinal Lymphangioma: A Case Report

もっと見る 閉じる

スポンサーリンク